Ku80 molecular weight

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August undefined, 2024

WebSynthetic peptide within Human Ku80 aa 700 to the C-terminus (internal sequence). The exact sequence is proprietary. Database link: P13010 Positive control Human tonsil tissue; HeLa cell lysate. ICC/IF: HeLa cells Flow Cyt: HeLa cells General notes This product is a recombinant monoclonal antibody, which offers several advantages including: Web1. To amplify the Ku80 constructs, transform 50–100 ng of plasmid into DH5α competent cells, and then plate on LB + AMP plate for overnight incubation at 37°C. Pick a single colony (choose small, not large colonies) and grow in 200 mL LB + AMP in a shaker incubator at 37°C for 24 h. Next day, purify the plasmid DNA by the Qiagen midi-prep kit …

Ku70 and Ku80 SpringerLink

Web1 mrt. 2001 · ATP-dependent DNA helicase 2 subunit KU80 EC number EC:3.6.4.12 ( UniProtKB ENZYME Rhea) Alternative names ATP-dependent DNA helicase 2 subunit … Web5 okt. 2024 · The Ku70–Ku80 (Ku) heterodimer binds rapidly and tightly to the ends of DNA double-strand breaks and recruits factors of the non-homologous end-joining (NHEJ) repair pathway through molecular ... sharninc

Ku80 removal from DNA through double strand break–induced ...

WebWhen using primary antibodies produced in mouse to detect proteins with a molecular weight in the range of 50 kDa, we recommend using Rabbit Anti-Mouse IgG (Light Chain … WebThe immunoprecipitated Ku80 protein was detected byab97433 at 1/1000 dilution, followed by anti-rabbit IgG antibody. IP Sample: 1000 μg HeLa (human epithelial cell line from … WebXRCC5/Ku80 Polyclonal Antibody for IF, IHC, IP, WB, ELISA Host / Isotype Rabbit / IgG Reactivity human, mouse and More (1) Applications WB, IP, IHC, IF, CoIP, ChIP, ELISA … sharnima jones chattanooga tn

Molecular characterization of KU70 and KU80 homologues and

WebSynthetic peptide within Human Ku80 aa 50-150. The exact sequence is proprietary. Positive control A549 (treated with FBS), HeLa, U937 and HepG2 cell lysates; human breast carcinoma tissue. General notes This product is a recombinant monoclonal antibody, which offers several advantages including: Web27 feb. 2014 · Comparison of the cDNAs with the genomic sequences revealed that the KU70 mRNA spans over 3,047 bp containing 16 exons separated by 15 introns, whereas the KU80 mRNA spans over 3,426 bp containing 11 exons separated by 10 introns (Figure 1 ). sharninWeb20 dec. 2016 · 'Ku70 and Ku80' published in 'Encyclopedia of Signaling Molecules' Studies showed that the Ku70/80 heterodimer is a key-player in the double-stranded DNA repair pathway nonhomologous end-joining (NHEJ) and binds to DNA termini with high affinity, where it helps to protect DNA ends from degradation and to recruit other NHEJ factors … sharni name meaning

"Web27 feb. 2014 · The Ku70 ORF sequence was predicted to encode for a protein of 706 amino acids with a molecular weight of 79.5 kDa. Ku70 showed 25% to 30% identities to those … " - Ku80 molecular weight

Ku80 molecular weight

Ku80 Antibody - Cell Signaling Technology

Web15 nov. 1997 · Mimori T, Akizuki M, Yamagata H, Inada S, Yoshida S and Homma M (1981) Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies from patients with polymyositis–schleroderma overlap. J Clin Invest, 68, 611 – 620. Crossref CAS PubMed Web of Science® Google Scholar Web丁香通为您找到232条80kd厂家的工商注册年份、员工人数、年营业额、信用记录、主营产品、相关80kd产品的供求信息、交易记录等企业详情。丁香通为买家提供用户服务，诚信保障等服务，批发采购80kd，上丁香通。

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Web25 mei 2007 · Mol. Weight (Da) 71222.7 Isoelectric Point 4.89 Median Abundance (molecules/cell) 1548 +/- 453 Alleles Curated mutant alleles for the specified gene, … Web15 nov. 2005 · Although Ku80 originally has been described as a nuclear protein, human bone marrow erythroid cells with glycophorin A or CD36, B cells with CD20, or T cells with CD3 were all positive for cell-surface expression of Ku80. B19 infection of KU812Ep6 cells and bone marrow cells was inhibited in the presence of anti-Ku80 antibody.

WebRecombinant Human Ku80 protein (ab132946) Datasheet. Submit a review Submit a question. $700 Product size. 2 µg. Add to basket. Order now and get it on Friday March … Web9 dec. 1999 · In the lysate of HeLa-S3 cells transformed with pEGFP-Ku80 (1 – 732), a strong signal from a protein of the expected molecular weight was detected by Western blotting using a rabbit...

Web28 jan. 2003 · Lane 1 shows molecular weight markers. Two prominent bands are indicated at 70 and 80 kDa that are precipitated with the antibody APC(N15) (lane 3), but that are not present in the control lane ... Web4 aug. 2008 · To monitor DNA-induced degradation, we added linear DNA to extract containing 35 S-labeled Ku80 at a concentration of 10 12 ends per microliter, or 0.6 mg/ml, in excess of the estimated concentration of Ku80 in the extract (0.7–2 × 10 11 molecules per microliter; Labhart, 1999).

Web3 jan. 2010 · Human Ku80 mRNA is stable in Xenopus oocytes post-microinjection Eighteen hours after the in vitro-transcribed mRNAs were injected into Xenopus oocytes that were metabolically labeled with 35 S-methionine, full length Ku70 polypeptide from the oocyte cytoplasm and nucleus could be radioimmunoprecipitated with the anti-T7 Tag antibody …

Web28 aug. 2024 · The number average molecular weight (M n) is given by 2.2.7 , where M i is molecular weight of a molecule of oligomer n, and N i is number of molecules of that molecular weight. Total weight = Σ ∞ i = 1MiNi. Total number = Σ ∞ i = 1Ni. Mn = Σ ∞ i = 1MiNi Σ ∞ i = 1Ni. Example 2.2.8. sharn in hindiWeb耐辐射球菌中RecA的调控蛋白研究 sharni norder twitterWeb26 dec. 2024 · Ku70 forms a heterodimer with Ku80, called Ku, that is critical for repairing DNA double-stand breaks by nonhomologous end joining and for maintaining telomeres. Mice with either gene mutated exhibit similar phenotypes that include increased sensitivity to ionizing radiation and severe combined immunodeficiency. However, there are also … sharni name originWeb17 apr. 2024 · Molecular Sciences Review A Comprehensive Analysis into the Therapeutic Application of Natural Products as SIRT6 Modulators in Alzheimer’s Disease, Aging, Cancer, Inﬂammation, and Diabetes Raushanara Akter 1,* , Afrina Afrose 1, Md. Rashidur Rahman 2, Rakhi Chowdhury 1, Saif Shahriar Rahman Nirzhor 3, Rubayat Islam Khan 4 and Md. … population of ontario and quebec canadaWeb5 dec. 2011 · All lanes : Anti-Ku80 antibody [EPR3468] (ab80592) at 1/50000 dilution Lane 1 : A549 cell lysate Lane 2 : HeLa cell lysate Lane 3 : HepG2 cell lysate Lane 4 : MCF7 … population of old town maineWeb14 apr. 2024 · Interaction between the BRCT domain of RAP1 and KU70/KU80 is also ... Molecular weights are displayed on the right. f Quantification of normalized telomeric DNA trapped in the well ... population of onslow county population of ontario id