WebJul 22, 2024 · IncHI 56 plasmids, often >200 kb in size, share a common core of approximately 160 kb. The differences 57 in size are due to the presence of distinct insertion elements, including many AMR determinants 58 [13]. IncHI-encoded AMR can be present in enterobacterial such as Salmonella, Escherichia coli WebComparative analysis of the pNDM-MAR sequence identified a novel type of IncH plasmid. This study underlines the diversity of genetic vehicles involved in the spread of the bla(NDM-1) gene. Plasmid pNDM-MAR differed significantly from all …
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WebJun 1, 2024 · Introduction. Plasmids of the H incompatibility (IncH) complex can be divided into two major Inc groups, IncHI and IncHII [1]. Incompatibility interactions between IncHI and IncHII can be explained by a negative control model: the IncHII repressor binds more strongly to the IncHI operator site than the IncHI repressor binds to the IncHII operator; … WebThe IncH family plasmids can produce the H-pilus, a long and flexible conjugation pilus similar to the F-pilus. Here, the pSIM-1-BJ01 possess two transfer regions, carrying the H-pilus assembly and transfer related protein genes ( trhL, trhE, traK, traB, dsbC, traV, traC, trhZ, trhO, htdA, htdK, traW, traU, traN, traD, traI, traF, traH and traG ).
WebJul 7, 2008 · IncHI plasmids are the only known plasmids in human pathogens that are temperature sensitive for transfer. Using R27 sequence data and transposon mutagenesis, the transfer determinants responsible for the spread of H-plasmids were identified ( Rooker et al., 1999; Sherburne et al ., 2000 ). WebDec 21, 2024 · It encoded 287 predicted proteins ( S1 Fig ). p2189-NDM belonged to the IncHI1 replicon type as it possessed IncHI1A-like and IncHI1B replicons with 99.8% and 100% similarity scores to IncHI1A and IncHI1B replicons of Salmonella Typhi plasmid R27 (accession no. AF250878), respectively.
WebIncHI plasmid conjugation is thermodependent and all transfer‐related genes are encoded in six operons (tra operons). Using R27, the prototype of IncHI1 plasmids, we reported that the plasmid‐encoded factor HtdA represses four of the six tra operons. WebJun 1, 2024 · IncHI plasmids are typically large, often >200 kb in size. Comparative analysis of some of the IncHI1 plasmids shows that these plasmids are very diverse in terms of genetic structure and have accumulated a number of accessory resistance regions [6], [7].
WebJul 1, 1982 · Salmonella typhi, in a patient with enteric fever, acquired two plasmids that together conferred resistance to the drugs used to treat the illness.One of them, of IncHI, conferred chloramphenicol and sulfonamide resistance and altered the phage type of the S. typhi.An indistinguishable plasmid was present in a strain of Klebsiella aerogenes …
WebMar 30, 2024 · IncHI plasmid is an important vector for the tet(X4) gene, which belongs to the H incompatibility (IncH) group, including IncHI1 to IncHI5 subgroups (Phan and Wain, 2008; Cui et al., 2024). The IncHI1 plasmid is a conjugative plasmid … flinders beach tide timesWebThe common regions of the IncHI plasmids contain genes that are essential for plasmid replication, maintenance, and transmission (which might define the general backbone for the group), as well as an extensive series of homologous core genes. Acquired DNA, that is not common among plasmids core, includes class 1 integrons, greater cleveland regional transit policeWebMay 15, 2000 · Abstract. Salmonella typhi, the causative agent of typhoid fever, annually infects 16 million people and kills 600 000 world wide.Plasmid-encoded multiple drug resistance in S.typhi is always encoded by plasmids of incompatibility group H (IncH). The complete DNA sequence of the large temperature-sensitive conjugative plasmid R27, the … flinders bay house augustaWebAug 19, 2024 · IncHI plasmids constitute a diverse collection of large (75–400Kb), self-transmissible, low-copy number mobile genetic elements commonly found in Gram negative Enterobacteriaceae ( Rozwandowicz et al., 2024 ). Molecular typing of IncHI plasmids provides discrimination between subgroups IncHI1, IncHI2, and IncHI3. flinders beach victoriaWebSep 17, 2024 · IncHI plasmids represent 20% of all plasmids transmitting colistin resistance worldwide and 40% in Europe. When analyzing the interactions of the IncHI1 plasmid R27 with Salmonella, we identified a large-molecular-mass protein that is encoded by this plasmid and is exported to the external medium. flinders beach camping qldWebGenome Sequence of the E. coli Isolate. The complete genome of the E. coli isolate 201,609 was 5.53Mb which contained a 5.20Mb circular chromosome and three resistance plasmids named pE648CTX-M-65, pE648NDM-5 and pE648MCR-1, respectively. The GC percentage of the genome is 50.2%. The average depth of the chromosome (the raw data/the assembled … greater cleveland rta bus mapWebAug 27, 2024 · The IncHI1 plasmid–positive strain 19CS0402 (p19CS0402-IncHI1) was sequenced using an Illumina MiSeq platform (Illumina Inc.), as well as with a MinION sequencer (Oxford Nanopore Technologies, Oxford, United Kingdom) to close sequences of the chromosome and the plasmid. flinders bowls club