WebNov 16, 2024 · Image processing was performed in RELION4 , unless otherwise specified. Dose-fractionated movies were motion-corrected with RELION’s implementation of the MotionCor2 algorithm , and contrast transfer function (CTF) parameters were estimated using CTFFIND4.1 (). After 2D classification in cryoSPARC v3.3.1 , 235,444 particles … WebApr 11, 2024 · Section snippets Structural organization of the junctional complex. To characterize the spectrin-actin junctional complex, we isolated the membrane skeleton from Sus scrofa domestica red blood cells (Figure S1). Through the optimization of cryo-EM sample preparation, we obtained images of a well-dispersed junctional complex (Figure …
[3dem] VPP phase development curve
WebApr 11, 2024 · The defocus values and astigmatism parameters for each micrograph were determined by CTFFIND4 41. In the micrographs, the B-capsid proteinaceous core allows for easy differentiation from the empty ... WebMar 13, 2024 · Package Details: ctffind4 4.1.5-1. Package Actions. View PKGBUILD / View Changes Download snapshot Search wiki Flagged out-of-date (2024-09-15) ... 1: Popularity: 0.000000: First Submitted: 2024-03-13 10:57 (UTC) Last Updated: 2024-03-13 11:55 (UTC) star ocean the divine force nihlbeth
ctffind4 The Grigorieff Lab
Webdata_dir/ is expected to contain a three-column TSV file of CTFFIND4 defocus values: (1) micrograph filename, (2) defocus x, and (3) defocus y. If this file is not found, then all micrographs will be assigned the same defocus value. A defocus file can be built from the output of a RELION CTF refinement job using the following Bash script: WebApr 1, 2014 · g++ -dpackage_name=\"ctffind\" -dpackage_tarname=\"ctffind\" -dpackage_version=\"4.1.14\" -dpackage_string=\"ctffind\ 4.1.14\" … WebFeb 27, 2024 · The cross correlation coefficient output from CTFFIND4 calculated within the resolution range of 10 ∼ 5 Å. Blue and red dots represent cross correlation coefficient of every micrograph after ... star ocean the divine force nexus